![]() Single-nucleus analysis of accessible chromatin in developing mouse forebrain reveals cell-type-specific transcriptional regulation. Integrative single-cell analysis of transcriptional and epigenetic states in the human adult brain. Multiplex single-cell profiling of chromatin accessibility by combinatorial cellular indexing. Simultaneous epitope and transcriptome measurement in single cells. Massively parallel single-nucleus RNA-seq with DroNc-seq. Massively parallel digital transcriptional profiling of single cells. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells. Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding. Comprehensive single-cell transcriptional profiling of a multicellular organism. Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput. Mapping the mouse cell atlas by Microwell-Seq. Single-cell epigenomics: recording the past and predicting the future. Revealing the vectors of cellular identity with single-cell genomics. Scaling single-cell genomics from phenomenology to mechanism. scGESTALT provides a scalable platform to map lineage relationships between cell types in any system that permits genome editing during development, regeneration, or disease. Generating transgenic lines takes 6 months, and performing barcode editing and generating single-cell libraries involve 7 d of hands-on time. Here, we provide details for (i) generating transgenic zebrafish (ii) performing multi-timepoint barcode editing (iii) building scRNA-seq libraries from brain tissue and (iv) concurrently amplifying lineage barcodes from captured single cells. The recorded lineages are captured, along with thousands of cellular transcriptomes, to build lineage trees with hundreds of branches representing relationships among profiled cell types. The technique generates edits in the barcode array over multiple timepoints using Cas9 and pools of single-guide RNAs (sgRNAs) introduced during early and late zebrafish embryonic development, which distinguishes it from similar Cas9 lineage-tracing methods. We recently established a method, scGESTALT, that combines cumulative editing of a lineage barcode array by CRISPR–Cas9 with large-scale transcriptional profiling using droplet-based single-cell RNA sequencing (scRNA-seq). concentration measurements in UV light) the colorless FastDigest Buffer is recommended.įor methylation sensitivity, refer to product specifications.Lineage relationships among the large number of heterogeneous cell types generated during development are difficult to reconstruct in a high-throughput manner. For applications that require product analysis by fluorescence excitation (e.g. Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. Restriction fragment length polymorphism (RFLP).100% buffer compatibility with downstream applications.100% activity of all FastDigest enzymes in the universal buffer.Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects. ![]() Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.įor additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels. ![]() DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. Thermo Scientific FastDigest NotI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers. Thermo Scientific FastDigest NotI restriction enzyme recognizes GC^GGCCGC site and cuts best at 37☌ in 5–15 minutes using universal FastDigest Buffer.
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Pages is the total number of pages visited by all visitors. For instance, visiting an HTML page that has two images on it generates three hits - one for the page itself and one for each image file.īandwidth downloaded is the total bandwidth used to download files. The “Monthly history” section is a 12-month graph that displays the same categories seen in the summary. The year-long view is interesting in that it demonstrates seasonal shifts in your traffic patterns. In general, web traffic slows down in the warmer summer months and increases in the cooler parts of the year. Depending on the topic of your website, you may see other kinds of seasonal shifts. These are similar to those surrounding television or sports seasons. Similarly, the “Days of month,” “Days of week,” and “Hours” sections can reveal visitor patterns that are useful in planning when you perform updates or publish new material. There is a treasure trove of other visitor statistics available in AWStats as well. ![]() Locales show a list of the top 25 countries in which your visitors are located. Again, this information is taken from IP addresses. That is because nothing in the server logs indicates the exact geographic location of a visitor. But for the most part, IP address information is a relatively accurate indication of location. Hosts (Top 25) are the IP addresses of your visitors. AWStats has already squeezed as much information as it can from the IP addresses and presented it to you in the other sections. User InformationĪuthenticated users (Top 10) is a list of the visitors who have accessed password protected directories. Robots/Spiders visitors (Top 25) is a list of the search engine spiders I mentioned above. Visits duration shows a list of time frames, and how many of your visitor sessions fell within those periods. ![]() ![]() If the majority of your visitors are only hanging around for a few seconds, you should consider adding more “long-form” content to keep them around. ![]() You can download the app from here, the app logo below, or scan the QR code with your iPad. To decrease the backlighting, press the Backlighting Level Down keyĪdditionally, you can update the keyboard firmware in the Logitech Control app. out door heaterphiladelphia riots iPad Pro 11 Apple Smart Keyboard Folio - I had it on a 3rd generation 11 Pro (not 100 sure its compatible across other. To increase the backlighting, press the Backlighting Level Up key You can adjust the backlighting of your iPad keyboard from 0% to 100% by 16 different levels. To enjoy the automatic adjustable backlighting feature and full backlight control features, you will need to update to iPadOS 13.6 or above. asian marvel movie Speck - Balance Folio R Case for Apple iPad Pro 11 (3rd/2nd/1st Gen) and iPad Air. How can I control the backlight of the Folio Touch iPad keyboard? iPad Pro, while the Logitech only has a variant. The following shortcut and media keys are available on the Folio Touch iPad keyboard: ![]() What are the shortcut keys on the Logitech Folio Touch iPad keyboard case? Yes the Folio Touch iPad keyboard will get deactivated when it is flipped around its back. Here’s how to connect your Folio Touch iPad keyboard:Īlign the three Smart Connector pins on your keyboard with the three Smart Connector pins on the backside of your iPad.Īfter you’ve aligned the Smart Connector pins, you’re connected and ready to type.ĭoes the Folio Touch iPad keyboard deactivate somehow when it is flipped around on the back? Or will the keys still press while holding it in that way? Apple Smart Keyboard Folio Case for 11-inch iPad Pro (3rd Generation), Black - US English. Apple Smart Keyboard Folio for iPad Air (4/5th gen) and iPad Pro 11 (3/4th gen) - Hungarian. When measured diagonally as a rectangle, the iPad Pro 12.9inch screen is 12.9 inches, the iPad Pro 11. Explore keyboards, cases, covers, Apple Pencil, AirPods, and more. Produkt: Klawiatura Apple do Apple iPad Pro 12,9 orygina. The Folio Touch uses the Smart Connector to connect to your iPad - it doesn’t require Bluetooth in order to pair. The Smart Keyboard Folio is a comfortable keyboard when you need one, and it provides front and back protection when you don’t. Apple Smart Keyboard Folio for iPad Pro 12,9-inch (3rd, 4th, 5th, 6th Gen) Stan. How do I pair the Folio Touch keyboard with my iPad? With two convenient viewing angles and no charging or pairing required, it’s simple to just attach the keyboard and start typing. The Folio Touch is compatible with iPad Pro 11-inch (1st, 2nd, 3rd & 4th gen) and Folio Touch for iPad Air (4th & 5th gen) The Smart Keyboard Folio for iPad Pro and iPad Air is a comfortable keyboard when you need one, and provides elegant front and back protection when you don’t. Is the Folio Touch iPad Keyboard case compatible with the latest iPad? ![]() ![]() Independent axles mean that the new self-leveling technology differs from a traditional boom lift with a solid oscillating axle in the front and a fixed axle in the rear. This is accomplished with four independent axles with intelligent sensing technology enabling drive at full height while actively leveling the chassis. The system allows the machine to traverse uneven surfaces while keeping the chassis level and improving traction capability on sloped ground. This increases a boom lift's capability on slopes from 0 to 10 degrees in any direction. The system provides a fully integrated solution to always make sure the machine automatically levels, even when driving the unit with the platform elevated. The self-leveling technology is designed to adjust the boom lift's chassis to the ground conditions (rather than adjust the ground conditions). These challenges were translated into requirements that defined the system architecture, including the mechanical, hydraulic and controls, as well as user interface design, with the goal of allowing the operator to traverse uneven ground with full functionality while driving at height. Boom lifts over a certain length and height can create a situation that requires special permits for shipping, such as being classified as an oversized load. In addition to determining the best method to provide a practical self-leveling experience for operators on different grades, it is crucial to provide the operator with easy and intuitive guidance while using a boom lift with this technology.Īnother one of the challenges that needs to be considered when designing a self-leveling chassis is staying within the dimensional limits for shipping on a truck. This traditional axle design offers minimal ability to adjust to uneven ground, often causing the boom to lean to the side and providing an uncomfortable operator experience, especially when driving at height. The solid axles in the front and back of a machine cannot provide the flexibility and adaptability needed when operating over uneven ground. To accomplish an intelligent suspension system that can traverse uneven ground and tackle slopes up to 10 degrees, it is crucial to think outside the box of a traditional boom lift. However, developing self-leveling technology comes with many design challenges that need to be overcome. Manual labor and time are often required to set up cribbing and/or grading the work area with earthmoving equipment.Īfter seeing how challenging boom lift applications could be, it became clear there is a need to develop a boom lift with a more adaptable suspension. This scenario is the root cause of many customer pain points on the jobsite since the world is neither flat nor does it come with improved surfaces. In addition, the new ANSI 92.20 standard in the United States requires boom functions to be cutout when the machine is placed on slopes greater than what they are rated for. Conventional machines are typically rated to work on a firm, level surface, which is usually defined as less than a 5-degrees of slope, yet most job sites aren't flat. Many applications and working environments can be challenging for a traditional boom lift to work in, even if equipped with an oscillating axle. ![]() Real-world job sites Self-leveling technology was developed due to watching how boom lifts are being used on job sites and talking with operators about their pain points and moments of inefficiency. JLG’s self-leveling chassis is a unique sub-system that allows a boom lift to automatically level on grades up to 10 degrees enabling operation on uneven jobsite terrain. One such technology is the development and integration of a Self-Leveling Chassis (SLC) for JLG® boom lifts. ![]() As the demand for increased jobsite efficiency continues to grow, MEWP (mobile elevating work platform) makers are forced to adopt, deploy and develop new technologies. Six weeks prior to presenting at our center, a transthoracic echo (TTE) showed normal left ventricular ejection fraction (LVEF) (60-65%) but the right ventricular systolic pressure (RVSP) was 38 mmHg. She had been diagnosed with pulmonary arterial hypertension (PAH) 6 weeks earlier, and was referred to our center for PAH evaluation. Case 1Ī 45 year-old African American female with seropositive rheumatoid arthritis (RA) for 8 years presented with intractable nausea, headaches, and dizziness to Emory University hospital in December, 2013. A represents case 1 and B represents case 2. Densitometer tracing of protein electrophoresis of patients’ serum. Paraprotein was not detected in either cases.įigure 1. Immunoglobulins G, A and M were elevated in both cases and both cases were negative for cryoglobulins. Both cases had positive rheumatoid factor and cyclic citrullinated peptide antibodies. Plasma viscosity was highest at presentation which was 8.4 for case 1 and 4.3 for case 2. Gamma globulins were higher in case 1 than in case 2 which was paralleled by the plasma viscosity. Figure 1 shows the serum protein electrophoresis at diagnosis of hyperviscosity syndrome for case 1 (Figure 1A) and case 2 (Figure 1B). Resultsīoth patients were African American females. The two cases met the American College of Rheumatology criteria for rheumatoid arthritis. We reviewed charts of two cases of rheumatoid HVS. This study was a prospective non-comparative clinical case series. In plasma cell dyscrasias, serum viscosity of 4-5cp, which corresponds to a serum IgM level of at least 3 g/dL, an IgG level of 4 g/dL, or an IgA level of 6 g/dL, has been associated with development of symptoms. The effectiveness of rituximab in B-cell depletion has been reported in other autoimmune diseases associated with immunoglobulin overproduction such as IgG4-related disease. Rituximab depletes B-cell precursors and mature B cells which express CD20, a cell surface protein, by binding to CD20 and increasing complement-dependent cytotoxicity, inducing antibody-dependent cell-mediated cytotoxicity by binding Fcy receptor on surface of effector immune cells, and by inducing non-classical apoptosis of B cells by crosslinking of multiple CD20 molecules. Rituximab is a mouse:human chimeric IgG1 anti-CD20 antibody which has been approved for treatment of rheumatoid arthritis by the United States Food and Drug Administration based on a large multicenter, randomized, double-blind, placebo-controlled study. Therapy that targets pathogenic B lymphocytes in rheumatoid HVS has not been reported previously. Although the first line of treatment of HVSfrom any cause is plasmapheresis, there are no studies to guide maintenance therapy. Only two cases of pulmonary arterial hypertension (PAH) leading to right heart failure have been reported. Congestive heart failure is uncommon in rheumatoid HVS. Patients usually present with non-specific symptoms such as epistaxis, bleeding gums, headaches, confusion, blurry vision, abdominal pain, fatigue, dyspnea, palmar erythema and stroke. The hallmark of HVS is polyclonal hypergammaglobulinemia associated with elevated serum viscosity leading to impairment of end organ perfusion. Hyperviscosity syndrome (HVS) is a rare extra-articular manifestation of rheumatoid arthritis with only 22 cases reported in literature. Hyperviscosity syndrome, rheumatoid arthritis, pulmonary arterial hypertension Introduction As far as we know, these are the first two cases of rheumatoid HVS which have been treated with rituximab. ![]() She was treated with prednisone followed by rituximab infusion. The second case was a post-partum cardiomyopathy which presented with inflammatory polyarthritis and compensated congestive heart failure. The case of pulmonary arterial hypertension was treated with two cycles of plasmapheresis followed by rituximab 1000 mg IV separated by 2 weeks and repeat dosing every 6 months. We report two cases of rheumatoid HVS who presented with either severe pulmonary arterial hypertension or post-partum cardiomyopathy. Rituximab is a powerful B cell depleting therapy which can theoretically eliminate pathogenic B cells and decrease immunoglobulin production in HVS. HVS is characterized by abnormal immunoglobulin production. Although plasmapheresis is effective as the first line treatment in life threatening manifestations such as pulmonary hypertension, stroke, retinal hemorrhages and congestive heart failure, data on effective maintenance immunosuppressive therapy is lacking. ![]() Hyperviscosity syndrome (HVS) is a rare manifestation of rheumatoid arthritis with few reported cases. ![]() The passphrase works with the key file to provide two-factor authentication. The passphrase can be empty but it's not recommended. You can press Enter to accept the default, or specify a path and/or filename where you would like your keys to be generated.Īt this point, you'll be prompted to use a passphrase to encrypt your private key files. The output from the command should display the following output (where "username" is replaced by your username): Generating public/private ed25519 key pair.Įnter file in which to save the key (C:\Users\username/.ssh/id_ed25519): ![]() To generate key files using the Ed25519 algorithm, run the following command from a PowerShell or cmd prompt on your client: ssh-keygen -t ed25519 A strong algorithm and key length should be used, such as Ed25519 in this example. If no algorithm is specified, RSA is used. ssh-keygen.exe is used to generate key files and the algorithms DSA, RSA, ECDSA, or Ed25519 can be specified. To use key-based authentication, you first need to generate public/private key pairs for your client. Since there's no user associated with the sshd service, the host keys are stored under C:\ProgramData\ssh. Get-Service -Name sshd | Set-Service -StartupType Automatic To start it each time the server is rebooted, run the following commands from an elevated PowerShell prompt on your server: # Set the sshd service to be started automatically Please see Getting started with OpenSSH.īy default the sshd service is set to start manually. You need to have OpenSSH Server installed first. The passphrase is used along with the presence of the private key on the SSH client to authenticate the user. The user will be prompted for the passphrase during authentication. Multi-factor authentication may be implemented with key pairs by entering a passphrase when the key pair is generated (see user key generation below). If the server-side public key can't be validated against the client-side private key, authentication fails. Key based authentication enables the SSH server and client to compare the public key for a user name provided against the private key. The public key is what is placed on the SSH server, and may be shared without compromising the private key. If someone acquires your private key, they can sign in as you to any SSH server you have access to. The private key files are the equivalent of a password, and should stay protected under all circumstances. SSH public key authentication uses asymmetric cryptographic algorithms to generate two key files – one "private" and the other "public". Key pairs refer to the public and private key files that are used by certain authentication protocols. If you're unfamiliar with SSH key management, we strongly recommend you review NIST document IR 7966 titled "Security of Interactive and Automated Access Management Using Secure Shell (SSH)". This document provides an overview of how to use these tools on Windows to begin using key-based authentication with SSH. scp and sftp to securely copy public key files during initial use of a server.ssh-agent and ssh-add for securely storing private keys. ![]() OpenSSH includes tools to help support key based authentication, specifically: When working across domains, such as between on-premises and cloud-hosted systems, it becomes vulnerable to brute force intrusions.īy comparison, Linux environments commonly use public-key/private-key pairs to drive authentication that doesn't require the use of guessable passwords. Most authentication in Windows environments is done with a username-password pair, which works well for systems that share a common domain. Applies to Windows Server 2022, Windows Server 2019, Windows 10 (build 1809 and later) ![]() It handles like a charm, making it the perfect crossover to handle your daily driving. Not your typical thrill-seeking SUV, but more than capable of having a grand time in West Long Branch. A unique coating on the Venue's pillars provides a buffer to reduce wind and road noise.Ī front-wheel driven powerhouse that is loaded with excitement and advanced technology. The standard eight-inch touchscreen provides a symmetrical feel to your cabin. ![]() A leather-wrapped shifter and steering wheel do not seem out of place in this affordable model. The standard cloth upholstery provides more class than your typical base model. No one should complain about their leg or headroom when riding inside. The cabin is deceptively large, considering the surroundings. Despite its diminutive stature, it very well could be one of the finest subcompact SUVs to take over the streets of Tinton Falls, in years. Quite simply the smallest SUV available from Asbury Park to Neptune. The 2020 Hyundai Venue gives Freehold drivers an affordable subcompact SUV option, for drivers who like the performance and utility a crossover provides without paying for the unwanted bulk. You can get your first look at Circle Hyundai in Shrewsbury. But unlike a lot of the other newcomers, the Hyundai Venue gives you something you won't find in many other rookie SUVs: a dynamic and unique look. Count the 2020 Hyundai Venue among the newest crossovers. In fact, it seems as if there are new crossovers hitting the streets every year. Hyundai Motor America reserves the right to amend, alter, or cancel this program, including its terms, at any time.There is a flood of SUV options sweeping across Red Bank and Middletown. Rates and reimbursements disclosed are subject to change without notice. Any recommended services and inspections outside of oil and oil filter changes, and tire rotations will be subject to charge. Does not cover services or repairs listed as “inspect” in the Owner’s Manual.Any additional maintenance due to severe driving is the customer’s responsibility. Normal wear and tear items such as engine and air filters, cabin filter, wiper blades, brake pads, etc. Only covers basic services listed in the Owner’s Manual at the time specified. ![]()
![]() Some features, applications and services may not be available in all regions or all languages. Available for homes using the new Home architecture and a HomePod or Apple TV running as a home hub.Join Testflight for iOS latest desktop client releases Talk Desktop Beta More ways to get involved Nextcloud offers a modern. After a major release it can take up to a month before it becomes available through the web installer and the updater. Available on iPhone 12 Pro, iPhone 12 Pro Max and later. Installer uses the same Nextcloud version as available for the built in updater in Nextcloud.Available on iPhone XS and iPhone XR and later and AirPods (2nd and 3rd generation), AirPods Pro (1st and 2nd generation) and AirPods Max, with the latest firmware.Here, you’ll see sections on Automatic Updates, and Beta Updates. ![]() Available in compatible applications with AirPods (3rd generation), AirPods Pro (1st and 2nd generation) or AirPods Max with the latest firmware. Open the iPhone’s Settings app, click on General, then Software Update. Download MPlayer OSX Extended for Mac now from Softonic: 100 safe and virus free.Available on iPhone XS and iPhone XR and later and AirPods Pro (2nd generation) with the latest firmware.Available in English on iPhone 12 and later.English, French and Spanish require iPhone 12 or later. Available in Arabic, Dutch, English, French, German, Hebrew, Korean, Italian, Polish, Portuguese, Romanian, Spanish and Thai. This guide helps you get started using iPhone and discover all the amazing things it can do with iOS 17.1, which is compatible with the following models: iPhone X R.Available on Apple Watch Series 7 or later, Apple Watch SE (2nd generation), and all Ultra models.Available on iPhone XS and iPhone XR and later and on Apple TV 4K (2nd generation and later).It is the final version of Office to be built exclusively for PowerPC and to officially support G3 processors, as its sequel lists a G4, G5, or Intel processor as a requirement. 178 It includes Microsoft Word, Excel, PowerPoint, Entourage and Virtual PC. Available on iPhone 12 and later when using the front camera. Microsoft Office 2004 for Mac was released on May 11, 2004. Featuresįind Any File has a few gems that other search tools do not offer:īetter results: It offers an alternative hierarchical view for the found items. Just download it now and pay later, if you want to keep using it. See the manual or simply try out FAF for free. See a video of Andy Ihnatko recommending FAF on the MacBreak Weekly 506 show Not convinced yet? Read this review of FAF at And with the option to include Spotlight results, it can also find text in PDF documents as long as they were indexed by Spotlight. FAF can also find textual content in plain text, in zip (including docx and xlsx) and even in most binary files.If you search for files by name, size, dates, kind and other directory properties, use FAF, because only then you can be sure that everything available is found, even if Spotlight hasn't indexed it.FAF cannot find metadata stored in files, such as image sizes, EXIF data and GPS coordinates (check out the alternatives below for that).įind Any File doesn't replace Spotlight, but it complements it greatly:.FAF cannot find text in PDF and other special document files (unless Spotlight has indexed them, which isn't necessarily the case on external and network disks).FAF lets you search precisely for many file properties such as name, extension, date range, size, kind etc.It can even search in other user's folders if you use FAF's unique root search mode. on network (NAS) and other external volumes, hidden ones inside bundles and packages, and those in folders that are usually excluded from Spotlight search, such as the System and Library folders. FAF can find files that Spotlight doesn't, e.g.Find Any File (FAF)įind Any File is a program for Apple's macOS that lets you search for files on your disks. This is useful if you want to try out new beta versions (available only here, not in the App Store see right side). You can download the version from this website and use it just the same, without having to purchase it again. Have you purchased FAF in the Mac App Store? Compatible with version 2 in regards to preferences and. ![]()
![]() The service will now use a number of factors, including real time traffic conditions and number of traffic lights, to make the proposed route safer, and more eco-friendly. It may ask if you want to take an alternate route depending on how bad the traffic gets, but it’s not particularly aggressive about it.īut Google Maps has changed the way it calculated its calculates its routes in recent years. Google Maps will flag traffic or obstacles it knows about on-screen, as does Waze, but the difference is Google will happily send you on the same pre-set route regardless of how things change. It's a running joke that Waze would tell you to drive through an active war zone to shave 5 seconds off your journey.īoth services can determine traffic conditions from the number of users on the road, but Waze actually does something about it. Rather than finding the quickest single path to your destination and leaving you to it, Waze is always analyzing conditions to try and get you there the fastest possible way. Waze, on the other hand, is built exclusively for car and motorbike users. Google can even alert you to obstacles or traffic along the way, and give you the option of taking a different path. You could walk, drive, cycle, or take the bus, and Google Maps has something to offer. You punch in a location, and it will find a way to get you there, no matter how you plan on doing it. Google Maps is your traditional navigation app. To the point where the “winner” depends entirely on how you get around. That being said, despite both being operated by Google, they are pretty different. Winner: Tie Google Maps vs Waze: NavigationĪt their core, both Google Maps and Waze will get you where you want to go. |
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